Proteinase activity regulation by glycosaminoglycans

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Proteinase activity regulation by glycosaminoglycans.

There are few reports concerning the biological role and the mechanisms of interaction between proteinases and carbohydrates other than those involved in clotting. It has been shown that the interplay of enzymes and glycosaminoglycans is able to modulate the activity of different proteases and also to affect their structures. From the large number of proteases belonging to the well-known protea...

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Cysteine cathepsins are a group of enzymes normally found in the endolysosomes where they are primarily involved in intracellular protein turnover but also have a critical role in MHC II-mediated antigen processing and presentation. However, in a number of pathologies cysteine cathepsins were found to be heavily upregulated and secreted into extracellular milieu, where they were found to degrad...

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Regulation of proBACEl by Glycosaminoglycans

The !3-secretase (BACE1) is initially synthesized as a partially act ive zymogen contain ing a prodomain which can be furthe r activated th rough proteolytic cleavage of the prodomain by a fu rin-Iike protease.The active site of BACEl is large and although a number of high-affinity active-site inh ibitors of BACEl have been described, most of these compounds are large, polar and do not cross th...

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Regulation of proteinase activity in Aspergillus nidulans.

1976) which is a defective in killer toxin secretion and in secretion of mature a-factor (T. Achstetter & D. H. Wolf, unpublished work). With the aid of the two model substrates, Cbz-Tyr-Lys and Cbz-Tyr-Lys-Arg, we also found carboxypeptidase activity in the membrane fraction, which is able to remove the two amino acids lysine and arginine and may thus be involved in further processing of the a...

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Cathepsin K, a lysosomal papain-like cysteine protease, forms collagenolytically highly active complexes with chondroitin sulfate and represents the most potent mammalian collagenase. Here we demonstrate that complex formation with glycosaminoglycans (GAGs) is unique for cathepsin K among human papain-like cysteine proteases and that different GAGs compete for the binding to cathepsin K. GAGs p...

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ژورنال

عنوان ژورنال: Brazilian Journal of Medical and Biological Research

سال: 2002

ISSN: 0100-879X

DOI: 10.1590/s0100-879x2002000200001